植物光合作包括光能的吸收、能量轉換、電子傳遞、ATP的合成以及CO2的固定等步驟，這一過程主要在植物的葉綠體的類囊體膜上完成的。類囊體膜上存在四種關鍵的蛋白復合體：光系統(tǒng)II（PS II）、Cytb6/f復合體、光系統(tǒng)I（PS I）和ATP合成酶，這四種復合體協(xié)同作用，共同完成光反應。光系統(tǒng)II上具有捕光色素復合體（LHC）構成的天線系統(tǒng)，可以捕獲光能，而光系統(tǒng)I則利捕獲后的能量進行原初光化學反應。Cytb6/f復合體在電子傳遞過程中起著至關重要的作用，ATP合成酶則負責合成ATP，為植物細胞提供能量。
|
貨號 |
實驗類型 |
|
|
ATP synthase |
||
|
AtpA | Alpha subunit of ATP synthase, chloroplastic |
AS08 304 |
IP,WB |
|
AtpB | Beta subunit of ATP synthase, chloroplastic + mitoch (rabbit) |
AS05 085 |
BN-PAGE,IF,WB |
|
AtpC | Gamma subunit of ATP synthase, chloroplastic |
AS08 312 |
WB |
|
Electron transfer |
||
|
Cyt b6/PetB | Thylakoid membrane cytochrome b6 protein, N terminal |
AS18 4169 |
BN-PAGE,WB |
|
Fd | Ferredoxin 1 (chloroplastic) |
AS11 1757 |
WB |
|
PetC | Rieske iron-sulfur protein of Cyt b6/f complex |
AS08 330 |
WB |
|
LHC |
||
|
Lhca1 | PSI type I chlorophyll a/b-binding protein |
AS01 005 |
WB |
|
Lhcb2 | LHCII type II chlorophyll a/b-binding protein |
AS01 003 |
IP,WB |
|
Lhcb3 | LHCII type III chlorophyll a/b-binding protein |
AS01 002 |
WB |
|
PSI (Photosystem I) |
||
|
PsaA | PSI-A core protein of photosystem I |
AS06 172 |
IG,WB |
|
PsaB | PSI-B core subunit of photosystem I |
AS10 695 |
WB |
|
PsaC | PSI-C core subunit of photosystem I |
AS10 939 |
WB |
|
PSII (Photosystem II) |
||
|
PsbA | D1 protein of PSII, C-terminal |
AS05 084 |
WB |
|
PsbB | CP47 protein of PSII |
AS04 038 |
WB |
|
PsbC | CP43 protein of PSII |
AS11 1787 |
WB |
AS09 522應用示例
AS06 172應用示例
Agrisera抗體發(fā)表文獻
Sulli et al. (2023). Generation and physiological characterization of genome?edited Nicotiana benthamiana plants containing zeaxanthin as the only leaf xanthophyll. Planta . 2023 Oct 5;258(5):93. doi: 10.1007/s00425-023-04248-3.
Ye et al. (2023). The light-harvesting chlorophyll a/b-binding proteins of photosystem II family members are responsible for temperature sensitivity and leaf color phenotype in albino tea plant. J Adv Res . 2023 Dec 25:S2090-1232(23)00404-6.doi: 10.1016/j.jare.2023.12.017.
Penzler et al. (2024). A pgr5 suppressor screen uncovers two distinct suppression mechanisms and links cytochrome b6f complex stability to PGR5. Plant Cell. 2024 Mar 27:koae098. doi: 10.1093/plcell/koae098.
Mu et al. (2024). Plastid HSP90C C-terminal extension region plays a regulatory role in chaperone activity and client binding.Plant J. 2024 Jul 5.doi: 10.1111/tpj.16917.
Rubisco
CO2的固定是利用光反應產生的ATP和NADPH合成有機物的過程，該過程稱為卡爾文循環(huán)。1,5二磷酸核酮糖羧化/加氧酶（Rubisco）是CO2固定過程中的關鍵限速酶，催化CO2和RuBP(核酮糖-1,5-二磷酸)發(fā)生羧化、然后裂解形成兩個PGA(甘油酸-3-磷酸)分子。所有光合生物都具有由8個大亞基RbcL和8個小亞基Rbcs組成的I型Rubisco，且RbcL折疊到其天然狀態(tài)依賴于葉綠體定位的分子伴侶CPN60的作用。
|
產品名稱 |
貨號 |
實驗類型 |
|
CAH3 | Carbonic anhydrase |
AS05 073 |
IF, IG, WB |
|
CPN60A1 | Chaperonin 60 subunit alpha 1, chloroplastic |
AS12 2613 |
WB |
|
CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C |
AS14 2760 |
WB |
|
EPSP synthase | 3-phosphoshikimate 1-carboxyvinyltransferase |
AS14 2782 |
WB |
|
EPYC1 | Essential Pyrenoid Component 1 |
AS19 4312 |
WB |
|
Lci5 | Low carbon dioxide induced protein number 5 |
AS05 090 |
IHC, WB |
|
NADP-ME | NADP-malic enzyme, chloroplastic (dicots/monocots) |
AS21 4580 |
WB |
|
PEPC | Phosphoenolpyruvate carboxylase |
AS09 458 |
IL, WB |
|
PEPCK | PEP carboxykinase |
AS07 241 |
WB |
|
PRK ribulose-5-P-kinase | Phosphoribulokinase |
AS07 257 |
WB |
|
RA | Rubisco activase |
AS10 700 |
WB |
|
RAF2 | Rubisco accumulation factor 2 |
AS13 2729 |
WB |
|
RbcL | Rubisco large subunit, form I (rabbit) |
AS03 037 |
IF, IG, TP, WB |
|
RbcL II | Rubisco large subunit, form II |
AS15 2955 |
IF, WB |
|
RbcS | Rubisco small subunit (SSU) |
AS07 259 |
WB |
|
RbcS | Rubisco small subunit (Algal) |
AS22 4825 |
WB |
|
Rubisco | 557 kDa hexadecamer |
AS07 218 |
IL, WB |
|
Rubisco ELISA quantitation kit |
AS15 2994 |
WB |
|
SBPase | Sedoheptulose-1,7-bis phosphatase |
AS15 2873 |
WB |
|
TKL1 | Transketolase (chloroplastic) |
AS15 2903 |
IP, WB |
|
Trxf1/2 | Thioredoxin F1/F2 (chloroplastic) |
AS14 2808 |
WB |
|
TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic) |
AS14 2809 |
WB |
應用案例
AS21 4580應用示例
AS03 037應用示例
Agrisera抗體發(fā)表文獻
Phukan et al. (2024). Externally supplied ascorbic acid moderates detrimental effects of UV-C exposure in cyanobacteria. Photochem Photobiol Sci. 2024 Jul 12. doi: 10.1007/s43630-024-00612-8.
Zhao et al. (2024). Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.Plant J. 2024 May 26. doi: 10.1111/tpj.16844.
Ciesielska et al. (2024). S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus of A. thaliana. Front Plant Sci. 2024 Mar 15:15:1372318. doi: 10.3389/fpls.2024.1372318.
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